Research Topic
CRISPR-Cas13a–Based Targeting of Antimicrobial Resistance Determinants in Multidrug-Resistant Klebsiella pneumoniae and Escherichia coli Isolates from Food-Producing Livestock in Ghana
Background
Antimicrobial resistance (AMR) is a growing threat to public health, food safety, and animal health worldwide. In Ghana, food-producing livestock increasingly serve as reservoirs of multidrug-resistant (MDR) bacteria, particularly Klebsiella pneumoniae and Escherichia coli. These organisms are commonly associated with zoonotic and foodborne infections and are capable of transferring resistance genes to humans through direct contact, contaminated animal products, and environmental exposure.
The widespread use of antibiotics in animal production has accelerated the emergence and spread of resistance genes that compromise the effectiveness of commonly used antimicrobials. Innovative and targeted approaches are therefore required to complement existing AMR control strategies, especially within the One Health framework.
Statement of the Problem
Despite national and regional efforts to address AMR, surveillance and intervention strategies in Ghana remain largely focused on detection rather than targeted mitigation of resistance at its source. MDR K. pneumoniae and E. coli circulating in food-producing livestock harbor clinically important resistance determinants that can enter the human population through the food chain.
However, there is limited laboratory-based research in Ghana exploring advanced, gene-targeted approaches for addressing resistance in animal reservoirs. This gap limits the development of effective, context-specific solutions to interrupt zoonotic transmission of AMR.
Research Objectives
To isolate and characterize multidrug-resistant K. pneumoniae and E. coli from food-producing livestock.
To determine phenotypic resistance profiles and identify key antimicrobial resistance genes.
To design CRISPR-Cas13a guide RNAs targeting selected resistance determinants.
To evaluate the ability of CRISPR-Cas13a systems to silence resistance genes and restore antibiotic susceptibility in vitro.
To assess biosafety, genetic stability, and potential off-target effects of the CRISPR-Cas13a approach.
Contribution to UN Sustainable Development Goals
Good Health & Well-being
Addressing antimicrobial resistance, a major threat to effective disease treatment globally.
Zero Hunger
Improving food safety and livestock health through targeted interventions.
Responsible Consumption & Production
Promoting prudent antimicrobial use in food production systems.
Significance of the Study
This study will generate laboratory-based evidence on AMR in zoonotic and foodborne pathogens from livestock, supporting One Health–driven AMR surveillance and control strategies in Ghana. By exploring CRISPR-Cas13a as a precision tool for targeting resistance genes, the research introduces innovative approaches that may complement conventional antimicrobial stewardship efforts.
The findings are expected to inform policy, strengthen research capacity, and contribute to national and regional AMR action plans.
Key Research Areas & Techniques
Expected Outcomes & Impact
Sponsorship & Collaboration Opportunities
The successful execution of this PhD research depends on access to advanced molecular biology and genomics facilities. I am actively seeking:
- Research sponsorship and funding partnerships
- Institutional partnerships (local or international)
- Laboratory hosting opportunities for sequencing and CRISPR experiments
- Biosafety analyses support
Collaborative laboratories and funding partners will play a critical role in enabling this work and advancing shared goals in AMR and One Health research.
Interested in Collaborating?
Let's work together to combat antimicrobial resistance
Jesse Azebiik Anak
PhD Candidate, Biotechnology
University of Ghana, Accra